Many herbal medicines and their active ingredients can effectively relieve asthma attacks and have become a hot spot for asthma treatment. In recent years, herbal medicine has received more and more attention due to its wide range of pharmacological effects, low toxicity, and few adverse reactions.
This treatment strategy has serious adverse reactions, such as headache, tremor, palpitations, and heart failure ( Bush et al., 2021 Jin et al., 2021). At present, the inhaled corticosteroids are the standard treatment for persistent asthma, but its antioxidant effect is not ideal ( Koh et al., 2021 Werder et al., 2021). Metabolomics combined with UPLC-MS is an integrated method to explore the metabolic mechanism of PF in the treatment of asthma rats and to reveal the potential targets, providing theoretical support for the study of the treatment of PF.Īsthma is characterized by chronic airway inflammation, airway hyperresponsiveness, and airway remodeling and is a serious public health problem in the world ( Chiu et al., 2021). The untargeted metabolomics combined with UPLC-MS is a useful tool for exploring the therapeutic mechanism and targets of PF in the treatment of asthma. In conclusion, we have discovered metabolite biomarkers and revealed the therapeutic mechanism of PF based on liquid chromatography coupled with mass spectrometry untargeted metabolomics. The key pathways including the citrate cycle (the TCA cycle), pyrimidine metabolism, pentose phosphate pathway, tyrosine metabolism, and tryptophan metabolism were affected by PF. A total of 20 potential biomarkers were discovered by UPLC/MS and urine metabolic profiling. Furthermore, high-throughput untargeted metabolic profiling was performed through ultraperformance liquid chromatography/electrospray ionization quadruple time-of-flight high-definition mass spectrometry (QA) UPLC-Q/TOF-MS combined with pattern recognition approaches and pathway analysis. In this study, we have explored the action mechanism of PF on asthma disease. However, its mechanism of action on asthma is still unclear. PF has gained a large amount of attention for its effect on asthma disease as the growth rate of asthma has increased in recent years. Paeoniflorin (PF) is a multi-target monoterpenoid glycoside and possesses broad pharmacological functions, e.g., anti-inflammation, anti-depression, antitumor, abirritation, neuroprotection, antioxidant, and enhancing cognitive and learning ability. 2Sanya Women and Children’s Hospital Managed by Shanghai Children’s Medical Center, Sanya, China.1Department of Pediatrics, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, China.Dan Wang 1 †, Li Zhao 2 †, Zhiyan Hao 1, Ying Huang 1, Yang Liao 1, Lingli Wang 1, Jinfeng Zhang 1, Shan Cao 1* and Lixiao Liu 1*
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